Protocol 1: Micro-Scale Isolation of Gangliosides

Stephan Ladisch - Children's National Medical Center

Solvent partition method for isolating gangliosides from small samples.

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Protocol 2: Immunohistochemistry

Tadashi Tai - Tokyo Metropolitan Institute of Medical Science

Immuno-fluorescence method for studying membrane and intracellular antigens.

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Protocol 3: Deglycosylation of Glycoproteins

Plummer & Tarentino - Wadsworth Center

Using endoglycosidases for glycoprotein deglycosylation.

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Protocol 4: ELISA-Based Glycosyltransferase Assay

Bruce A. Macher - San Francisco State University

Detect and quantify glycosyltransferase activity without radiolabeled substrates.

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Protocol 5: Immunostaining TLC of Glycolipids

John L. Magnani - GlycoTech Corporation

Detect carbohydrate ligands of protein receptors on TLC plates.

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Protocol 6: High Performance Liquid Affinity Chromatography

WeiTong Wang - GlycoTech Corporation

Analyze oligosaccharide ligands and determine binding constants.

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Protocol 7: Dynamic Flow Assay

John T. Patton - GlycoTech Corporation

Cell adhesion visualization under defined wall shear stress.

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Protocol 8: Static Cell Adhesion Measurement

John L. Magnani - GlycoTech Corporation

96-well plate assay using gravity-based wash chamber.

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Protocol 9: Cell Adhesion to Glycolipids on TLC

Ronald L. Schnaar - Johns Hopkins University

Detect specific cell adhesion to resolved glycolipids.

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Technical Reference Use

These protocols are provided as technical reference material for trained laboratory personnel. Results may vary. End users are responsible for validating all methods for their specific research applications. These procedures are not intended for clinical diagnostic use.