Apparatus Catalog

Parallel Plate Flow Chambers

Human Neutrophils Rolling on
Human Endothelial Cells Expressing E-selectin

Parallel plate flow chambers are used in dynamic studies of cell adhesion under well-defined shear forces. Various gasket sizes allow flexibility in experimental design to operate with limited quantities of cells and/or reagents.
(Refer to Protocol 7)

Circular Flow Chamber (for 35mm tissue culture dishes)

31-001
Circular Flow Chamber Kit ($675.00)

Technical Reference: Improvements to Parallel Plate Flow Chambers to Reduce Reagents and Cellular Requirements, Brown and Larson, BMC Immunology 2001, 2:9

Flow Chamber Kit Includes:

Two flow decks
(cast acrylic with different inlet/outlet designs)

8 silicon rubber gaskets (two of each size)
Flow Width = 0.25 cm, Thickness = 0.005 in
Flow Width = 0.25 cm, Thickness = 0.010 in
Flow Width = 0.50 cm, Thickness = 0.010 in
Flow Width = 1.00 cm, Thickness = 0.010 in

24 polypropylene tubing connectors (six of each)
Threaded 1/16 in. straight and L-connectors
Male and Female taper luer to 1/16 in. connectors

Silastic™ laboratory tubing (1/16 in. ID x 10 ft.)

Replacement Gaskets and Coverslips for the Circular Flow Chamber

31-002 Four Gaskets (Flow Width = 0.25 cm, Thickness = 0.005 in) $30.00

31-003 Four Gaskets (Flow Width = 0.25 cm, Thickness = 0.010 in) $30.00

31-004 Four Gaskets (Flow Width = 0.50 cm, Thickness = 0.010 in) $30.00

31-005 Four Gaskets (Flow Width = 1.00 cm, Thickness = 0.010 in) $30.00

31-008 Circular Glass Coverslips (pack of 30, 35 mm, #2) $30.00

Rectangular Flow Chamber (for standard microscope slide)

31-010
Rectangular Flow Chamber Kit
$625.00

Flow Chamber Kit Includes:

One flow deck (cast acrylic)

4 silicon rubber gaskets (two of each size)
Flow Width = 1.00 cm, Thickness = 0.005 in
Flow Width = 1.00 cm, Thickness = 0.010 in

24 polypropylene tubing connectors (six of each)
Threaded 1/16 in. straight and L-connectors
Male and Female taper luer to 1/16 in. connectors

Silastic™ laboratory tubing (1/16 in. ID x 10 ft.)

Replacement Gaskets for the Rectangular Flow Chamber

31-011 Four Gaskets (Flow Width = 1.0 cm, Thickness = 0.005 in) $30.00

31-012 Four Gaskets (Flow Width = 1.0 cm, Thickness = 0.010 in) $30.00